evaluation of mouse oocyte mitochondrial distribution in different developmental stages using confocal microscopy

Images of mitochondrial UCP 1 in mouse thymocytes using confocal microscopy.

The aim of this study was to demonstrate the constitutive expression of mitochondrial uncoupling protein 1 (UCP 1) in pure thymocytes using laser scanning confocal microscopic imagery. To that end we probed thymocytes from UCP 1 knock-out and wild-type mice. Mitochondrial location in thymocytes was determined using Mitotracker Red and the nucleus was labelled using Hoescht stain. We demonstrate...

P-99: Evaluation of Pluripotency Markers of Mouse Endometrial Tissue in Different Stages of Estrous Cycle

Background: It is assumed that adult stem/progenitor cells are responsible for cycling remodeling of the uterus endometrium throughout the reproductive life of the female. This study aimed to identify and localize stem/progenitor cells in the mice uterus using immunohistochemistry. Materials and Methods: 6-8 weeks old virgin female NMRI mice were submitted to the vaginal smear examination to de...

Determination of experimental Tumor Volume of a mouse using optical imaging system in different processing stages

Proteome of mouse oocytes at different developmental stages.

The mammalian oocyte possesses powerful reprogramming factors, which can reprogram terminally differentiated germ cells (sperm) or somatic cells within a few cell cycles. Although it has been suggested that use of oocyte-derived transcripts may enhance the generation of induced pluripotent stem cells, the reprogramming factors in oocytes are undetermined, and even the identified proteins compos...

Evaluation of topical cysteamine therapy in the CTNS−/− knockout mouse using in vivo confocal microscopy

PURPOSE The purpose of this study was to assess the ability of quantitative in vivo confocal microscopy (CM) to detect changes in cystine crystal volume in the cystinosisn (Ctns(-/-))mouse cornea following topical cysteamine therapy. METHODS Fifteen Ctns(-/-) mice were sequentially followed using in vivo CM from 3 to 10 months of age. In a second experiment, five mice receiving topical cystea...

Mapping Cell Distribution in Amorphous Scaffolds using Confocal Microscopy

INTRODUCTION One common tissue engineering (TE) approach for regenerating or replacing damaged tissues involves a porous polymeric scaffold that serves as the mechanical framework for cell attachment and proliferation. Cell-material interactions play a vital role in the structure and function of a scaffold and are affected by chemical and physical parameters, as well as the micro-environment su...